第12期涂知明等:酶标探针在转基因植物检测中的应用1537Ming-Jie.Geneticanalysisofthetransgenictritordeum.forblothybridization,EMBOJ,1983,2:817?822.JournalofHuazhongUniversityofScienceandTechnol-[6]RenzM,KurzC.AcolorimetricmethodforDNAhybridi-ogy(NatureScience),2005,33(8):94?96.zation.NucleicAcidsResearch,1984,12:3435?3438.何光源,吴旭乾,涂知明,陈明洁.转基因tritordeum[7]TUZhi-Ming,KELi-Hua.StudyoftheenzymelabeledHBVDNAprobe.VirologicaSinica,1991,6(2):的遗传分析.华中科技大学学报(自然科学版),2005,132?137.33(8):94?96.涂知明,柯丽华.酶标HBVDNA探针的研制及应用.[2]CHENLn,HEGuang-Yuan,TUZhi-Ming,CHENMing-Jie.Pollenspecificexpressionanalysisofthetransgenic病毒学杂志,1991,6(2):132?137.tritordeum.JournalofHuazhongUniversityofScience[8]CHENMing-Jie,LIUYong,TUZhi-Ming,HEandTechnology(NatureScience),2006,34(4):116?118.Guang-Yuan.Rapidconfirmationofthetransgenicwheat陈泠,何光源,涂知明,陈明洁.tritordeum花粉特异性linesbymultiplexpolymerasechainreaction(MPCR).JournalofHuazhongUniversityofScienceandTechnol-表达的遗传分析.华中科技大学学报(自然科学版),ogy(NatureScience),2004,32(9):105?107.2006,34(4):116?118.陈明洁,刘勇,涂知明,何光源.多重PCR法快速鉴定[3]LIUZhong,YANGGui-Zhen,WANGWen-Yu.ExploringofhybridizationconditionofthebiotinlabeledDNAprobe.转基因小麦植株及后代.华中科技大学学报(自然科学ProgressofBiochemistryandBiophysics,1992,19(6):版),2004,32(9):105?107.478?481.[9]SambrookJ,FritschEF,ManiatisT.MolecularClone:A刘钟,杨贵贞,王文余.生物素标记DNA探针杂交条件LaboratoryManual(2ndedition),ColdSpringHarbor探讨.生物化学与生物物理进展,1992,19(6):478?481.LaboratoryPress,1995.[4]WUYun-Tao,FANGQing.ProgressofthepreparationJ.撒姆布鲁克,E.F.弗里奇,T.曼尼阿蒂斯著(金冬雁,黎techniquesofnucleicacidprobes.ForeignMedicine(Mo-孟枫等译,侯云德等校).分子克隆实验指南(第二版).lecularBiologyEdition),1989,11(3):101?104.北京:科学出版社,2002.吴云涛,方勤.核酸分子探针制备技术新进展.国外医[10]ZMTu,LHKe,GYHe.Theapplicationofalkalinephos-学分子生物学分册,1989,11(3):101?104.phataselabeledHBVprobeinserumdetection.Virus[5]RenzM.Polynucleotide-histoneH1complexesasprobesGenes,2004,28(2):153?158.?ˇˇ?ˇ?ˇ?ˇˇ??ˇ?ˇˇ?ˇ??ˇˇ??ˇ?ˇˇ??ˇ?ˇˇ??ˇˇ??ˇˇ???ˇˇ??ˇˇ??ˇˇ?ˇˇ??ˇ?ˇˇˇ??ˇ?ˇ?ˇ?ˇˇ??ˇ?ˇˇ??ˇˇ??ˇˇ??ˇˇ?ˇ?ˇ?ˇ?ˇˇ??ˇ?ˇˇ?ˇ??ˇ?ˇˇ??ˇˇ??ˇ?ˇˇ?ˇ?ˇ??ˇ?ˇ?ˇ?ˇˇ??ˇ?ˇˇ?ˇ??ˇ?ˇˇ??ˇ?ˇˇ??ˇ?ˇˇ?ˇ?ˇ?ˇˇ??ˇˇ??ˇ??ˇˇ??ˇˇ??ˇˇ